Flow cytometry assessment of intracellular BTK expression

Flow Cytometry Analysis of Intracellular Protein

The demand for determining protein levels in a continuously decreasing amount of input cells is currently being developed by scientists and medical doctors. Another demanding area is the splitting of active and inactive (phosphorylated and unphosphorylated, respectively) forms of the protein being monitored, e.g., during different phases of the cell cycle, differentiation or carcinogenesis. The...

Comprehensive assessment of chemokine expression profiles by flow cytometry.

The chemokines are a large family of mainly secreted molecules involved in the regulation of numerous physiological and pathophysiological processes. Despite many years of investigation, the precise cellular sources of most chemokines have remained incompletely defined as a consequence of the limited availability of suitable reagents to visualize the expression of chemokine proteins at the sing...

assessment of different permeabilization methods of minimizing damage to the adherent cells for detection of intracellular rna by flow cytometry

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

assessment of helicobacter pylori viability by flow cytometry

background: flow cytometry is a rapid, sensitive, and reliable method for determination of bacterial viability. here we assayed the capability of flow cytometry to detect helicobacter pylori viable cells in both forms of spiral and coccoid. methods: viable bacteria stained with rhodamin 123 and fluoresced with laser beam of 488nm. the rate of rh123 absorption was determined in both forms of bac...

Intracellular Flow Cytometry - Multiparameter analysis of cytokine, transcription factor, and phosphoprotein expression